| Equipment for NAWQA ground water and surface water studies (updated May 2005) | ||||||||
| SW | GW | Qty. | Suppliera | Product/Model Number | Estimated price (each) | Description | ||
| NON-EXPENDABLE EQUIPMENT | ||||||||
| x | 500 mL polypropylene sample bottles | pack (12) | Fisher | 02-896E | $38.16 | autoclavable, for bacteria sample collection and transport | ||
| x | x | 1liter polypropylene sample bottles | case (6) | VWR | 16126-134 | $113.04 | autoclavable, for bacteria sample collection and transport | |
| x | 3 liter polypropylene sample bottles | case (6) | Fisher | 11-825D | $85.00 | autoclavable, for coliphage sample collection and transport | ||
| x | x | alcohol burner | 2 | Hach | 20877-42 | $19.10 | fill with methanol for flame sterilizing forceps | |
| x | x | alcohol well | 2 | Hach | 23240-43 | $3.60 | containing methanol for forceps | |
| x | beakers - 250 mL, optional | 2 | Hach | 500-46H | $4.70 | for melting agar. An autoclave may also be used. | ||
| x | x | autoclavable squirt bottle for buffer (500 mL), optional | pack (6) | Fisher | 03-409-14A | $30.51 | to dispense buffer during filtration | |
| x | x | autoclave - large (only one autoclave is needed)b | 1 | VWR | 58700-008 | $7,908.09 | for sterilization, capable of maintaining 121.6°C | |
| x | x | autoclave - small (only one autoclave is needed)b | 1 | Fisher | 02-964-1 | $2,874.12 | for sterilization, capable of maintaining 121.6°C | |
| x | x | Fluke 51 digital thermometer | 1 | Grainger | 4YV88 | $169.00 | for checking temperature of field incubators | |
| x | x | NIST thermometer | 1 | Fisher | 15-041C | $188.53 | range of -1 to 101°C, National Institute of Standards and Technology | |
| x | x | HOBO temperature logger | 1 | Onset | H01-001-01 | $49.00 | temperature recording device to monitor incubators | |
| x | x | HOBO subcase | 1 | Onset | SUBC2-WH | $29.00 | used with HOBO temperature logger | |
| x | x | dual-chamber incubator | 1 | Millipore | XX632K115 | $3,040.00 | capable of maintaining temperature ranges for incubation | |
| x | x | forceps | 2 | Fisher | 10-300 | $3.51 | stainless steel, smooth tips | |
| x | x | gloves | 1 pair | Fisher | 11-394-299 | $16.86 | for removal of items from autoclave | |
| x | x | graduated cylinders | case (8) | Fisher | 08-570-21D | $58.86 | autoclavable, for measuring sample volumes | |
| x | pipet bulbs | 3 | Fisher | 13-681-51 | $12.09 | SW, use to deliver sample; GW, use to prepare cefsulodin soln. | ||
| x | x | tally counter | 2 | Fisher | 07-905-6 | $27.75 | hand-held for counting bacterial colonies | |
| x | hot plate (with magnetic stirrer) for media prep, optional | 1 | Fisher | 11-496-8 | $434.23 | for melting agar. An autoclave may also be used. | ||
| x | x | Leica Zoom 2000 Stereoscope (Model Z45L) c | 1 | VWR | 41433012 | $993.00 | wide-field for viewing colony growth, 10-20 X magnification | |
| x | x | Portable illuminated magnifiersc | 1 | VWR | 36934-107 | $88.00 | for viewing colony growth, 7 X mangification | |
| x | x | manifold | 1 | VWR | 28145-305 | $625.00 | for holding filtration units during filtration | |
| x | x | membrane filtration units (for GW, addn'l units may be nee | 3 | VWR | 28143-550 | $158.00 | autoclavable, for filtering samples | |
| x | x | peristaltic pump (should already have for GW sampling) | 1 | GSA | 81350004 | $688.50 | vacuum source, not to exceed 5 psi | |
| x | x | pump head-easy load (should already have for GW sampli | 1 | GSA | 81350021 | $190.00 | needed to run pump | |
| x | x | tubing for filter flask/manifold: Masterflex sizes 15 | 1 | GSA | 87050000 | $2.12/ft | to connect manifold to peristaltic pump ($2.12/ft for 25 ft) | |
| x | longwave ultraviolet lamp (366nm) | 1 | Spectronics Corp. | EN-160L | $122.00 | for use with the MI method (total coliforms) | ||
| x | UV lamp viewing box | 1 | Spectronics Corp. | CM-10 | $179.00 | for use with the MI method (total coliforms) | ||
| EXPENDABLE EQUIPMENT/SUPPLIES | ||||||||
| x | x | 1 mL pipets, sterile, to deliver (TD)f | case (1000) | Fisher | 13-678-11B | $103.36 | sterile, disposable, used to deliver sample | |
| x | 10 mL pipets, sterile, to deliver (TD) | case (200) | Fisher | 13-678-11E | $35.83 | sterile, disposable, used to deliver sample | ||
| x | 25 mL pipets, sterile, to deliver (TD) | case (200) | Fisher | 13-676-10K | $93.99 | sterile, disposable, used to deliver sample | ||
| x | x | aluminum foil for sterilization (15 in. by 1000 ft.) | 1 box | GSA | 8135-00-724-0552 | $44.34 | for maintaining sterility of equipment after autoclaving | |
| x | x | autoclave bags | pack (200) | Fisher | 01-830A | $34.47 | for disposal of used plates and other contaminated items | |
| x | ethanol | 1 gallon | VWR | RC65901 | $30.20 | for sterilizing the spigot on sampling wells | ||
| x | x | handsoap | 2 bottles | VWR | 56614-420 | $18.29 | for washing hands | |
| x | x | Liquinox | 1 bottle | Fisher | 04-322-15A | $10.85 | for cleaning dishes | |
| x | x | marking pens, color-coded tape | as needed | Fisher | approx. | $30.00 | labeling petri dishes, buffer, and dilution bottles | |
| x | x | membrane filters - 0.45 mm pore size, 47mm diameter | 2 boxesd | Advantec | A045H047A | $24.00 | sterile, with grids, disposable | |
| x | x | methanol | 1 liter | VWR | EM-MX0475-1 | $47.32 | for use with burner | |
| x | 9x50mm sterile, perti dishes | 3 boxesd | Advantec | 800100 | $17.51 | sterile, plastic, disposable | ||
| x | BD
dehydrated MI agar (One of two suppliers for MI agar) |
bottle (100g) | Gov't Scientific | 214882 | $255.00 | media for determination/enumeration of E. coli and total coliforms | ||
| x | MI
agar (pre-poured plates) (One of two suppliers for MI agar) |
100 plates | Fisher | B14985 | $313.00 | media for determination/enumeration of E. coliand total coliforms; cefsulodin is already added to the plates | ||
| x | Millex Syringe Filters, sterile - 0.22mm pore size, 33mm dia.f | box (50) | Fisher | SLGP033RS | $86.89 | used to sterilize cefsulodin solution before addition to MI agar | ||
| x | 3cc Syringe, sterilef | pack (100) | Fisher | SCH1144 | $13.91 | used to sterilize cefsulodin solution before addition to MI agar | ||
| x | Cefsulodin (100mg)f | bottle | Sigma | C8145 | $24.00 | antibiotic used in MI agar | ||
| x | Microcentrifuge tubes (1.5mL)f | pack (500) | Fisher | 02681-458 | $20.52 | autoclavable, used in sterilization of cefsulodin solution for MI agar | ||
| x | modified
mTEC agar (pre-poured plates)g (One of two suppliers for mod. mTEC agar) |
275 platesd | Hach | 28118-01 | $3.00 | media for determination/enumeration of E. coli | ||
| x | BD
modified mTEC dehydrated agar (One of two suppliers for mod. mTEC agar) |
bottle (100g) | Gov't Scientific | 214884 | $309.96 | media for determination/enumeration of E. coli | ||
| x | dilution blanks PO4/MgCl2 (99 mL bottles) | 24 | Hardy Diagnostics | D699 | $0.41 | for preparing a dilution series | ||
| x | x | sterile buffered water PO4/MgCl2 (250 mL bottles) | 24 | Hardy Diagnostics | U193 | $3.89 | buffer water for rinsing and quality control samples | |
| x | x | rubbing alcohol for benchtop cleanup | 1 bottle | grocery store | - | $2.00 | for cleaning work surfaces | |
| x | x | biological indicators (spore tests) for autoclave QC | 3 packs | Fisher | 14-490-25 | $17.54 | quality control: to monitor sterilization capabilities of autoclave | |
| x | x | sterilization tape | pack (12) | VWR | 36432-186 | $39.53 | quality control: to monitor sterilization capabilities of autoclave | |
| x | x | Escherichia coli | pack (10) | Remel | 4751985 | $207.25 | Positive control culture for NAWQA SW and GW | |
| x | x | Enterobacter cloacae | pack (10) | Remel | 4757090 | $207.25 | Positive control cultures for NAWQA GW/ Negative control culture for NAWQA SW | |
| x | Pseudomonas aeruginosa | pack (10) | Remel | 4757060 | $207.27 | Negative control cultures for NAWQA GW | ||
| aThe list of supplier is for your convenience. Other suppliers may be used. | ||||||||
| b A large autoclave is preferred but is more expensive than a small one and requires proper installation. The small autoclave is suitable for SW sampling where only 1-L bottles are used; for GW, 3-L sample bottles are needed for coliphage and only one can fit at one time in the small autoclave. | ||||||||
| cAlthough the Stereoscope is preferred, the Portable illuminated magnifier is an adequate substitute. | ||||||||
| d Quantity specified is suitable for approximately 30 GW or 45 SW samples and related QC. | ||||||||
| e Perishable - order as needed. Recommended holding time for the USGS is 45 days after receipt (although manufacturer states a holding time of 3 months). | ||||||||
| f For GW studies: items required when using BD dehydrated MI agar only. | ||||||||
| g Hach plates are presently produced on plates that are too large to fit in old blue incubators. If you purchase the new incubator listed above, plates will fit okay. If you have the old blue incubators, you need to obtain plates from a custom order for the USGS on the smaller plates. Contact the Ohio District about arranging for a custom order. | ||||||||
| Advantec 800.334.7132 | Millipore 800.645.5476 | |||||||
| Fisher 800.766.7000 | Onset Computer Corporation 508.759.9500 | |||||||
| GSA 800.525.8027 | Remel Inc. 800.447.3635 | |||||||
| Government Scientific Source, Inc. 800.248.8030 | Sigma-Aldric | |||||||
| Grainger 888.361.8649 | Spectronics | |||||||
| Hach 800.227.4224 | USGS - Ohio 614.430.7700 | |||||||
| Hardy Diagnostics 800.266.2222 | VWR 800.932.5000 | |||||||