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OWML: QA/QC

Quality Assurance/Quality Control Manual: Ohio Water Microbiology Laboratory.
Updated January 2014

By Donna S. Francy, Rebecca N. Bushon, Amie M. G. Brady, Christopher M. Kephart, Erin A. Stelzer, and Christopher D. Ecker

ABSTRACT

INTRODUCTION

GENERAL LABORATORY QUALITY ASSURANCE/QUALITY-CONTROL PRACTICES

METHODS OF ANALYSIS, MEDIA AND REAGENT PREPARATION, AND ANALYTICAL QUALITY-CONTROL PROCEDURES

 

REFERENCES


APPENDICES

General Instructions

Forms

Culture Methods

Molecular methods (appendices are available upon request)

  • D1. Collection and initial processing of water samples for MST markers and Cyanobacteria
  • D1a. Bench sheet for filtration and extraction for MST and Cyanobacteria
  • D2. Collection and initial processing of solid samples for MST markers and Cyanobacteria
  • D2a. Field sheet for collection of known-source fecal samples
  • D3. DNA extraction and purification using the GeneRite DNA-EZ kit
  • D3a. Extraction batch bench sheet
  • D4. Detection of MST markers and Cyanobacteria by qPCR
  • D4a. qPCR bench sheets for MST assays
  • D4b. qPCR bench sheets for Cyanobacteria DNA
  • D4c. RT-qPCR bench sheets for Cyanobacteria RNA
  • D5. Development and quantification of plasmid positive controls
  • D5a. Plasmid-based positive control bench sheet
  • D6. qPCR data interpretation and file management

Enteric virus methods (appendices are available upon request)

  • E1. Virus elution and organic flocculation
  • E1a. Virus processing form
  • E1b. Virus qPCR batch sheet
  • E2. Molecular concentration and extraction
  • E3. Reverse transcription and qPCR protocol for viruses
  • E3a. qPCR and RT-qPCR benchsheets for viruses
  • F1. Total culturable virus assay
  • F1a. Total culturable virus and viral titering benchsheet

Tables

   

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