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Quality Assurance/Quality Control Manual: Ohio Water Microbiology Laboratory

Research Methods

Direct detection of pathogens
The Ohio Water Microbiology Laboratory (OWML) has adapted methods for the detection/enumeration of Bacillus anthracis, Burkholderia cepacia, Cryptosporidium parvum, Francisella tularensis, and Vibrio cholerae using qPCR. Ultrafiltration is being used for the concentration of these organisms from water (Morales-Morales and others, 2003).

The OWML has established protocols using qPCR for enumeration of adenovirus (Jothikumar and others, 2005a) and quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) for enumeration of enterovirus (Gregory and others, 2005) and norovirus GI (Jothikumar and others, 2005b). Glass-wool filtration (Lambertini and others, 2008) and ultrafiltration are being used for the concentration of viruses from water.