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OWML: QA/QC

Quality Assurance/Quality Control Manual: Ohio Water Microbiology Laboratory
 

Research Methods

Microbial source tracking markers
Microbial source tracking using molecular markers is carried out by detecting genetic sequences in the DNA of fecal-origin bacteria that are specific to the host species that produced the feces. Host-specific molecular markers have been identified based on the theory that the physiology in the gut of the host animal (e.g. diet, temperature, antibiotic treatment, etc.) is unique from one animal to another. These unique conditions select for unique subsets of microorganisms in the gut. Numerous host-specific markers have been developed for various host animals. The Ohio Water Microbiology Laboratory (OWML) has adopted the capability to analyze for the following markers:

Marker

Source

Reference

AllBac

General

Layton and others, 2006

GenBac

General

Siefring and others, 2008

BoBac

Bovine

Layton and others, 2006

qHF183

Human

Seurinck and others, 2005

BacHum

Human

Kildare and others, 2007

Gull2

Gull

Lu and others, 2008

BacCan

Dog

Kildare and others, 2007

LA-35

Chicken

Weidhass and others, 2010