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OWML: QA/QC

Quality Assurance/Quality Control Manual: Ohio Water Microbiology Laboratory

 

Table 6.  Information on media, buffered-dilution water, and reagents prepared and stored in the Ohio Water Microbiology Laboratory (OWML).

TYPE OF MEDIA/BUFFER

SOURCE

STORAGE

HOLDING TIME

mENDO agar

Fisher Scientific or Hardy Diagnostics

Dry agar – Cabinet

Plates - refrigerator

As specified by manufacturer

5 days as plates

MI agar

OWML

Refrigerator

6 months in dilution bottles

2 weeks as plates

MI agar

Govern. Scientific Source or Fisher Scientific

Dry agar – Cabinet

Plates - refrigerator

As specified by manufacturer

2 weeks as plates

Colilert

Idexx Corp., Westbrook, ME

Cabinet

As specified by manufacturer

 

mFC agar

Fisher Scientific or Hardy Diagnostics

Dry agar – Cabinet

Plates - refrigerator

As specified by manufacturer

3 days as plates

Modified mTEC

OWML

Refrigerator

6 months in dilution bottles

2 weeks as plates

Modified mTEC

Govern. Scientific Source or Fisher Scientific

Dry agar – Cabinet

Plates - refrigerator

As specified by manufacturer

 

mEI

OWML

 

Refrigerator

 

6 months in dilution bottles

3 days to 2 weeks as plates*

mEI Govern. Scientific Source or Fisher Scientific Dry agar - Cabinet Plates - Refrigerator As specified by manufacturer
2 weeks as plates
Enterlolert Idexx Corp., Westbrook, ME Cabinet As specified by manufacturer

mCP agar

OWML

Refrigerator

6 months in dilution bottles

1 month as plates

mCP agar Oxoid Dry agar - Cabinet As specified by manufacturer

Phosphate buffer with magnesium chloride (Appendix A2)

OWML

 

Cabinet (unopened)

Refrigerator (after opening)

1 year (unopened)

1 week (after opening)

Phosphate buffer with magnesium chloride

Hardy Diagnostics, CA

Cabinet (unopened)

Refrigerator (after opening)

As specified by manufacturer

 

 

* If reagents that are added after autoclaving are filter sterilized, the longer holding time is applied.