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OWML: QA/QC

Quality Assurance/Quality Control Manual: Ohio Water Microbiology Laboratory

 

Table 7: Target pH values for media and reagent preparation

Media/Reagent

Final pH

+/-

Reference

Actinomycete Isolation Agar

8.10

0.2

Difco #212168 Bottle

Bile Esculin Agar

6.80

0.2

USEPA; Method 1600; EPA-821-R-06-009; 2006

Brain Heart Infusion Agar

7.40

0.2

USEPA; Method 1600; EPA-821-R-06-009; 2006

Brain Heart Infusion Broth

7.40

0.2

USEPA; Method 1600; EPA-821-R-06-009; 2006

EC Broth

6.90

0.2

USEPA; Method 1603; EPA-821-R-06-011; 2006

ISP Media 1

7.00

0.2

Difco #276910 Bottle

ISP Media 2

7.20

0.2

Difco #277010 Bottle

mCP Agar*

7.60

not stated

Bisson and Cabelli (1979)

mE Agar

7.10

0.2

Difco #233320 Bottle

mEI Agar**

7.10

0.2

USEPA; Method 1600; EPA-821-R-06-009; 2006

mEndo Agar

7.20

0.2

Difco #273620 Bottle

mEndo Broth MF

7.20

0.1

Difco #274930 Bottle

mFC Agar

7.40

0.2

Difco #267720 Bottle

MI Agar***

6.95

0.2

USEPA; Method 1604; EPA-821-R-02-024; 2002

MI Broth

7.05

0.2

USEPA; Method 1604; EPA-821-R-02-024; 2002

mod mTEC Agar

7.30

0.2

USEPA; Method 1603; EPA-821-R-06-011; 2006

Nutrient Agar

6.80

0.2

USEPA; Method 1603; EPA-821-R-06-011; 2006

Phosphate Buffered Saline

7.40

0.2

USEPA; Method 1603; EPA-821-R-06-011; 2006

Simmons Citrate Agar

6.90

0.2

USEPA; Method 1603; EPA-821-R-06-011; 2006

Tryptic Soy Agar

7.30

0.2

USEPA; Method 1604; EPA-821-R-02-024; 2002

Tryptic Soy Broth

7.30

0.2

USEPA; Method 1603; EPA-821-R-06-011; 2006

Tryptone

7.30

0.2

USEPA; Method 1603; EPA-821-R-06-011; 2006

Working Phosphate Buffered Dilution Water

7.00

0.2

USEPA; Method 1603; EPA-821-R-06-011; 2006

*pH adjust before autoclaving

 

 

 

**Note: pH after nalidixic acid and TTC addition (aliquot a portion to check the pH)

 

***Note: pH after cefsulodin addition (aliquot a portion to check the pH)

 

 

Updated 10/1/08