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QPCR Rapid Detection Method

What is the QPCR rapid method?

 

The quantitative polymerase chain reaction (QPCR) method enumerates targeted genetic sequences within microorganisms in less than three hours.  First, water samples are concentrated either by passing through a 0.4 m filter, if targeting bacteria, or by ultrafiltration through a hollow-fiber ultrafilter, if targeting viruses.  The concentrated organisms are lysed by both physical and chemical disruption and the genetic material that was contained in these organisms is then isolated and purified.  The QPCR is run and the genetic sequence unique to the organism of interest is quantified by detecting the accumulation of a fluorescent probe.

 

 

 

 

 

 

Current Ohio Water Science Center Projects Currently Testing the QPCR Method