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QPCR Rapid Detection Method

Ohio Water Science Center Projects Currently Testing the QPCR Method.

In cooperation with the Northeast Ohio Regional Sewer District, QPCR is being used for rapid detection of E. coli for assessing recreational water quality. The QPCR method was tested during the recreational seasons of 2006 and 2007 at two Lake Erie beaches near Cleveland, Ohio—Edgewater and Villa Angela. Water samples were analyzed by both the traditional 24-hour culture-based method and by QPCR. Results are to be published in 2008. To learn more about this project, click here. Geese - QPCR Rapid Detection Method Project.

Chris Kephart - QPCR Rapid Detection Methods Project. QPCR is being used to test for six pathogens that are considered potential biological warfare agents. In cooperation with the U.S. Environmental Protection Agency, National Homeland Security Research Center, drinking water samples are being spiked with six pathogens and then analyzed by both the traditional cultural or microscopic methods and by QPCR. The efficiency and variability of recoveries of these methods are being determined. For more information about this project, click here.

The Ohio Water Microbiology Laboratory is currently working on building the capability to analyze water samples for enteric viruses by QPCR. Funding from the USGS Instrumentation Committee has allowed for testing and optimization of QPCR for three enteric viruses—adenovirus, enterovirus, and norovirus. Ultrafiltration using a hollow-fiber ultrafilter is being tested as a means for concentrating large volumes of water. Click here for more information about this project. Centricon - QPCR Rapid Detection Methods Project.