USGS Ohio Water Science Center

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Ohio Water Microbiology Lab

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Ohio Water Science Center

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USGS Water Science Centers are located in each state.

There is a USGS Water Science Center office in each State. Washington Oregon California Idaho Nevada Montana Wyoming Utah Colorado Arizona New Mexico North Dakota South Dakota Nebraska Kansas Oklahoma Texas Minnesota Iowa Missouri Arkansas Louisiana Wisconsin Illinois Mississippi Michigan Indiana Ohio Kentucky Tennessee Alabama Pennsylvania West Virginia Georgia Florida Caribbean Alaska Hawaii New York Vermont New Hampshire Maine Massachusettes South Carolina North Carolina Rhode Island Virginia Connecticut New Jersey Maryland-Delaware-D.C.

IMS/ATP Rapid Detection Method

What is the IMS/ATP rapid method?

IMS/ATP Rapid Detection Method. The immunomagnetic separation/adenosine triphosphate (IMS/ATP) rapid method requires approximately 1 hour from sample collection to availability of results. Magnetic beads that are coated with antibodies for either Escherichia coli (E. coli) or enterococci are added to a water sample. This mixture is then subjected to IMS, in which the bacteria-antibody-bead complex is separated from extraneous materials in the sample by use of a strong magnet. Following several wash/concentration steps, the bacterial cells are ruptured by an enzymatic process, releasing ATP, which is the energy molecule found in living cells. The amount of ATP in the sample is measured with a microluminometer and results are reported in relative light units (RLUs).

 

>Current Ohio Water Science Center Projects Currently Testing the IMS/ATP Method


Original publication of the method can be found at:

Lee, JiYoung, and Deininger, R.A., 2004, Detection of E. coli in beach water within 1 hour using immunomagnetic separation and ATP bioluminescence: Luminescence, v. 19, p. 31–36.

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