USGS Ohio Water Science Center

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Ohio Water Microbiology Lab

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Ohio Water Science Center

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USGS Water Science Centers are located in each state.

There is a USGS Water Science Center office in each State. Washington Oregon California Idaho Nevada Montana Wyoming Utah Colorado Arizona New Mexico North Dakota South Dakota Nebraska Kansas Oklahoma Texas Minnesota Iowa Missouri Arkansas Louisiana Wisconsin Illinois Mississippi Michigan Indiana Ohio Kentucky Tennessee Alabama Pennsylvania West Virginia Georgia Florida Caribbean Alaska Hawaii New York Vermont New Hampshire Maine Massachusettes South Carolina North Carolina Rhode Island Virginia Connecticut New Jersey Maryland-Delaware-D.C.


Quality Assurance/Quality Control Manual: Ohio Water Microbiology Laboratory

Research Methods

Direct detection of pathogens
The Ohio Water Microbiology Laboratory (OWML) has adapted methods for the detection/enumeration of Bacillus anthracis, Burkholderia cepacia, Cryptosporidium parvum, Francisella tularensis, and Vibrio cholerae using qPCR. Ultrafiltration is being used for the concentration of these organisms from water (Morales-Morales and others, 2003).

The OWML has established protocols using qPCR for enumeration of adenovirus (Jothikumar and others, 2005a) and quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) for enumeration of enterovirus (Gregory and others, 2005) and norovirus GI (Jothikumar and others, 2005b). Glass-wool filtration (Lambertini and others, 2008) and ultrafiltration are being used for the concentration of viruses from water.


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