USGS Ohio Water Science Center

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Ohio Water Microbiology Lab

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Ohio Water Science Center

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USGS Water Science Centers are located in each state.

There is a USGS Water Science Center office in each State. Washington Oregon California Idaho Nevada Montana Wyoming Utah Colorado Arizona New Mexico North Dakota South Dakota Nebraska Kansas Oklahoma Texas Minnesota Iowa Missouri Arkansas Louisiana Wisconsin Illinois Mississippi Michigan Indiana Ohio Kentucky Tennessee Alabama Pennsylvania West Virginia Georgia Florida Caribbean Alaska Hawaii New York Vermont New Hampshire Maine Massachusettes South Carolina North Carolina Rhode Island Virginia Connecticut New Jersey Maryland-Delaware-D.C.

OWML: QA/QC

Quality Assurance/Quality Control Manual: Ohio Water Microbiology Laboratory

 

Table 6.  Information on media, buffered-dilution water, and reagents prepared and stored in the Ohio Water Microbiology Laboratory (OWML).

TYPE OF MEDIA/BUFFER

SOURCE

STORAGE

HOLDING TIME

mENDO agar

Fisher Scientific or Hardy Diagnostics

Dry agar – Cabinet

Plates - refrigerator

As specified by manufacturer

5 days as plates

MI agar

OWML

Refrigerator

6 months in dilution bottles

2 weeks as plates

MI agar

Govern. Scientific Source or Fisher Scientific

Dry agar – Cabinet

Plates - refrigerator

As specified by manufacturer

2 weeks as plates

Colilert

Idexx Corp., Westbrook, ME

Cabinet

As specified by manufacturer

 

mFC agar

Fisher Scientific or Hardy Diagnostics

Dry agar – Cabinet

Plates - refrigerator

As specified by manufacturer

3 days as plates

Modified mTEC

OWML

Refrigerator

6 months in dilution bottles

2 weeks as plates

Modified mTEC

Govern. Scientific Source or Fisher Scientific

Dry agar – Cabinet

Plates - refrigerator

As specified by manufacturer

 

mEI

OWML

 

Refrigerator

 

6 months in dilution bottles

3 days to 2 weeks as plates*

mEI Govern. Scientific Source or Fisher Scientific Dry agar - Cabinet Plates - Refrigerator As specified by manufacturer
2 weeks as plates
Enterlolert Idexx Corp., Westbrook, ME Cabinet As specified by manufacturer

mCP agar

OWML

Refrigerator

6 months in dilution bottles

1 month as plates

mCP agar Oxoid Dry agar - Cabinet As specified by manufacturer

Phosphate buffer with magnesium chloride (Appendix A2)

OWML

 

Cabinet (unopened)

Refrigerator (after opening)

1 year (unopened)

1 week (after opening)

Phosphate buffer with magnesium chloride

Hardy Diagnostics, CA

Cabinet (unopened)

Refrigerator (after opening)

As specified by manufacturer

 

 

* If reagents that are added after autoclaving are filter sterilized, the longer holding time is applied.

   

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