USGS Ohio Water Science Center

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Ohio Water Microbiology Lab

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Ohio Water Science Center

USGS In Your State


USGS Water Science Centers are located in each state.

There is a USGS Water Science Center office in each State. Washington Oregon California Idaho Nevada Montana Wyoming Utah Colorado Arizona New Mexico North Dakota South Dakota Nebraska Kansas Oklahoma Texas Minnesota Iowa Missouri Arkansas Louisiana Wisconsin Illinois Mississippi Michigan Indiana Ohio Kentucky Tennessee Alabama Pennsylvania West Virginia Georgia Florida Caribbean Alaska Hawaii New York Vermont New Hampshire Maine Massachusettes South Carolina North Carolina Rhode Island Virginia Connecticut New Jersey Maryland-Delaware-D.C.

QPCR Rapid Detection Method

What is the QPCR rapid method?

 

The quantitative polymerase chain reaction (QPCR) method enumerates targeted genetic sequences within microorganisms in less than three hours.  First, water samples are concentrated either by passing through a 0.4 m filter, if targeting bacteria, or by ultrafiltration through a hollow-fiber ultrafilter, if targeting viruses.  The concentrated organisms are lysed by both physical and chemical disruption and the genetic material that was contained in these organisms is then isolated and purified.  The QPCR is run and the genetic sequence unique to the organism of interest is quantified by detecting the accumulation of a fluorescent probe.

 

 

 

 

 

 

>Current Ohio Water Science Center Projects Currently Testing the QPCR Method


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